Ana Mansourkiaei
Islamic Azad University, Iran
Title: Phylogenetic Relationship analysis of Scomberomorus commerson in Persian Gulf, Oman Sea and Arabian Sea and Thunnus tonggol in Oman Sea using mitochondrial DNA D-Loop
Biography
Biography: Ana Mansourkiaei
Abstract
Narrow-barred Spanish mackerel (Scomberomorus commerson) and Long tail tuna (Thunnus tonggol) are epipelagic and migratory species of family Scombridea which have a significant role in terms of ecology and fishery. Phylogenetic relationships of S. commerson from the Persian Gulf and Oman Sea and Arabian Sea, and T. tonggol from Oman Sea were investigated through sequence data of mitochondrial DNA D-loop region using High Resolution Melting-Real Time PCR. S. commerson were classified into 20 groups and T. tonggol were banded into 8 groups. Finally 40 samples of S. commerson and 16 samples of T. tonggol were chosen to sequence for phylogenetic and genetic analyses. None clustered NJ tree indicated the proximity amid S. commerson in four stations and T. tonggol into stations. As figures demonstrated in sequence analyses of mitochondrial DNA D-Loop region a sublimely high degree of genetic similarity among S. commerson from the Persian Gulf and Oman Sea and Arabian Sea and also among T. tonggol from the Oman Sea were perceived, thereafter, having one stock structure of S. commerson in four regions and one stock structure of T. tonggol in two regions were proved. And this approximation can be merely justified by their emigration process along the coasts of Oman Sea and Persian Gulf and Arabian Sea Accordingly, The assessment of distribution patterns of 40 samples of S. commerson and 16 samples of T. tonggol in the constructed Phylogenetic tree and using mtDNA D-Loop sequences ascertained that no significant clustering according to the sampling sites were concluded. And Fst values shows this similarity as well.